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Hersteller: Agilent Technologies Dateigröße: 262.21 kb Dateiname: spinconcentratorsinstructions.pdf Unterrichtssprache:

2 Place concentrator into 50 mL centrifuge tube (filtrate collection vessel – not supplied, user must provide). 3 Insert assembled concentrator into centrifuge (when fixed angle rotors are used, angle the concentrators so that the printed windows face upwards/outwards). Spin at recommended speeds, taking care not to exceed the maximum g force indicated in the table above (20-40 minutes is common). 4 Once the desired concentration is achieved, remove assembly and recover sample from the bottom of the concentrate pocket with a pipette. Desalting/Buffer Exchange See 4 mL Spin Concentrator instructions on the reverse side. Notes: . Spin concentrators are single use disposable items. Re-use is not recommended. . For research use only. . Select the most appropriate membrane cut-off for your sample. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest. . These concentrators are designed for use with biological fluids or aqueous solutions only, not for organic solvents or strong acids or bases. . Flow rates are affected by MWCO, porosity, sample size, concentration, viscosity, centrifuge force, and temperature. Adjust centrifuge force and time accordingly for your samples. . Do not autoclave. Sterilize with a 70% ethanol solution. For more information or technical assistance, Agilent Technologies, Inc. please call toll free: 1-800-227-9770 or visit 2850 Centerville Road our web site at: Wilmington, DE 19808-1610 USA 05/2004A ...

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